Comparative analysis of hyperuricemia induction methods and probiotic interventions in mice

Area of Science:

• Metabolic disorders
• Microbiology
• Renal physiology

Background:

• Hyperuricemia (HUA) management requires standardized animal models, as current methods show significant variability.
• Probiotics are functional food additives with potential therapeutic benefits, but their role in HUA mitigation needs further investigation.

Purpose of the Study:

• To systematically compare methods for inducing HUA in mice.
• To investigate the efficacy of probiotics in regulating uric acid levels and mitigating HUA-related renal impairment.

Main Methods:

• Comparison of different HUA induction methods (adenine, inosine, guanosine, hypoxanthine) in C57BL/6JNifdc mice.
• Screening and evaluation of seven probiotic strains for uric acid degradation and effects on uric acid metabolism in vitro and in vivo.
• Assessment of renal function, hepatic enzyme activity, and uric acid transporter protein expression.

Main Results:

• Adenine induced severe renal injury and altered enzyme activity, while other methods caused milder HUA.
• Probiotics demonstrated significant uric acid degradation capabilities.
• Probiotics modulated renal uric acid transporter expression and inhibited renal inflammation.

Conclusions:

• Selecting appropriate animal models is crucial for HUA research.
• Specific probiotics show therapeutic potential for managing hyperuricemia by degrading uric acid, modulating renal transporters, and reducing inflammation.