M^Ⅱ-polyphosphate-based wax-like material with osteogenesis and blood occlusion

Area of Science:

• Biomaterials Science
• Orthopedic Surgery
• Regenerative Medicine

Background:

• Current hemostatic materials in orthopedic surgery often lack biodegradability and osteogenic properties.
• There is a clinical need for materials that provide both hemostasis and osteogenesis for bone repair.

Purpose of the Study:

• To develop and evaluate a novel wax-like co-doped polyphosphate (CoPPW) material for combined hemostatic and osteogenic effects.
• To assess the degradation profile, ion release, in vitro cellular response, and in vivo bone regeneration capabilities of CoPPW.

Main Methods:

• CoPPW was synthesized by chelating sodium polyphosphate with Ca2+, Mg2+, and Sr2+ ions.
• In vitro studies involved cell proliferation and osteogenic differentiation assays, RT-PCR, and transcriptome sequencing.
• In vivo studies compared CoPPW with bone wax in promoting bone regeneration and hemostasis.

Main Results:

• CoPPW demonstrated optimal malleability and degradation over 4 weeks, releasing Mg2+ and Sr2+ ions.
• In vitro, CoPPW significantly enhanced cell proliferation and osteogenic differentiation, upregulating key genes and proteins via the PI3K/AKT pathway.
• In vivo, CoPPW exhibited superior bone regeneration and hemostasis compared to traditional bone wax, activating the coagulation cascade.

Conclusions:

• CoPPW possesses excellent hemostatic and osteogenic properties, surpassing conventional bone wax.
• The PI3K/AKT pathway activation and coagulation cascade indicate effective osteogenesis and hemostasis, respectively.
• CoPPW is a promising candidate for orthopedic applications requiring combined bone repair and bleeding control.