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Published on: September 4, 2012
Engineered Exosomes Delivering bta-miR-223 Alleviate Staphylococcus aureus-Induced Mastitis in Mice via the
Yuxuan Wang1, Kun Ouyang1, Ying Nie1
1College of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou, Guangdong 510642, China.
None:
Bovine mastitis is an inflammatory disease of the mammary gland, frequently associated with infection by Staphylococcus aureus. Effective delivery of the antiinflammatory microRNA bta-miR-223 remains a challenge. In this study, we constructed engineered exosomes loaded with bta-miR-223 and demonstrated their antiinflammatory effects both in vitro (Mac-T cells) and in vivo (mice). Overexpression of bta-miR-223 reduced the expression of IL-6 and IL-1β in a lipoteichoic acid-induced Mac-T cell inflammation model. Following tail vein injection in lactating mice, the engineered exosomes accumulated in the mammary gland, alleviated S. aureus-induced inflammation, and increased the expression of barrier-related proteins ZO-1, claudin-1, and occludin. Mechanistically, bta-miR-223 inhibited RHOB expression and modulated the TLR4/NF-κB pathway. These results demonstrate that exosome-mediated delivery of bta-miR-223 effectively alleviates mammary inflammation, providing a novel strategy for nucleic acid nanotherapy.
