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Host antigens associated with haemagglutinin
Vaccine
|September 1, 1985
Summary
A delayed-type hypersensitivity (DTH) test identified host antigens in influenza virus preparations. Lipid-containing subunits triggered DTH responses, while lipid-free components did not, indicating the importance of lipid envelopes in antigen presentation.
Area of Science:
- Immunology
- Virology
- Biochemistry
Background:
- Influenza virus contains host-specific antigens.
- Understanding antigen presentation is crucial for vaccine development.
Purpose of the Study:
- To investigate the role of viral components in eliciting delayed-type hypersensitivity (DTH) responses.
- To detect host-specific antigens associated with influenza virus and its components.
Main Methods:
- Utilized a DTH test in cyclophosphamide-treated mice.
- Primed mice with allantoic fluid extracts and challenged with various influenza virus preparations (lipid-containing subunits, lipid-free derivatives, monomer hemagglutinin).
- Extended the approach to SV40-vector expressed hemagglutinin.
Main Results:
- DTH reactions were observed when mice were challenged with preparations containing envelope lipid or polymer-type subunits.
- Lipid-free derivatives, including internal virus protein complexes and monomer hemagglutinin, did not elicit DTH responses.
- Associated host antigens were detected in SV40-vector expressed hemagglutinin from simian cells.
Conclusions:
- The lipid envelope of influenza virus plays a critical role in eliciting DTH responses.
- DTH testing is a viable method for detecting host-specific antigens in viral preparations.
- This methodology can identify host antigens in recombinant viral proteins.