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Updated: Sep 17, 2025

Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation
Published on: October 31, 2019
Conventional versus emerging techniques in probiotic enumeration: a comprehensive review
Xiaoyun Sun1, Juan Zhang1, Jiaci Chen1
1Institute of Quality Standards and Testing Technology for Agro-Products, State Key Laboratory for Quality and Safety of Agro - Products, Chinese Academy of Agricultural Sciences, Beijing, China.
Abstract:
Probiotics are beneficial microorganisms that support host health. They are extensively used in food, healthcare and pharmaceutical industries. Accurate viability assessment is imperative not only to ensure product quality and efficacy but also to meet consumer expectations. This review provides a systematic analysis of probiotic history, international regulatory standards and functional characteristics across strains. It provides a comprehensive overview of probiotic enumeration techniques, which range from traditional culture methods to modern emerging technologies that drive current research and applications. By critically evaluating the strengths and limitations of these methods, this review explores future directions for probiotic enumeration, offering valuable insights for quality control in probiotic products and supporting advancements in related scientific research.
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