Core-shell microbead-based 3D vascularized glioma tumor model for effective drug testing
Xiuxiu Zhang1, Zixian Wang2, Zeyang Liu3
1Precision Medicine and Healthcare Research Center, Tsinghua-Berkeley Shenzhen Institute (TBSI), Tsinghua University, Shenzhen 518055, People's Republic of China.
Biofabrication
|July 3, 2025
Summary
This study developed a 3D vascularized tumor model using core-shell microbeads. This advanced model better mimics in vivo tumors for more accurate drug testing and mechanistic studies.
Area of Science:
- Biomedical Engineering
- Cancer Research
- Drug Development
Background:
- 3D hydrogel tumor models show promise for in vivo replication.
- Mimicking vascularized, compartmentalized microtumors remains challenging for high-throughput studies.
- Existing models lack controlled heterogeneity and accurate physiological representation.
Purpose of the Study:
- To engineer a vascularized 3D tumor model using core-shell microbeads.
- To incorporate an endothelial cell (EC) barrier for improved in vivo mimicry.
- To evaluate the model's utility in drug testing and mechanistic studies.
Main Methods:
- Encapsulation of glioma cells (core) and human umbilical vein endothelial cells (HUVECs) (shell) in core-shell microbeads (gelatin/GelMa).
- Induction of EC barrier formation via HUVEC migration and adhesion.
- Assessment of angiogenesis, spheroid formation, and drug response (doxorubicin).
Main Results:
- The engineered microbeads formed functional EC barriers and vascularized tumor spheroids.
- The 3D model accurately replicated in vivo tumor structure and properties.
- Vascularized tumor models exhibited significantly higher doxorubicin IC50 values than 2D cultures.
Conclusions:
- The developed vascularized 3D tumor model effectively mimics in vivo tumor microenvironments.
- This microengineered system offers a powerful platform for preclinical drug screening and mechanistic investigations.
- Engineered 3D tumor models hold significant potential for advancing cancer research and drug development.
