Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Esterase activities and soman toxicity in developing rat.

S H Sterri, G Berge, F Fonnum

    Acta Pharmacologica Et Toxicologica
    |August 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The fungal neurotoxin penitrem A induces the production of reactive oxygen species in human neutrophils at submicromolar concentrations.

    Toxicology·2017
    Same author

    Non-dioxin-like PCBs inhibit [(3)H]WIN-35,428 binding to the dopamine transporter: a structure-activity relationship study.

    Neurotoxicology·2013
    Same author

    Mechanisms of penitrem-induced cerebellar granule neuron death in vitro: possible involvement of GABAA receptors and oxidative processes.

    Neurotoxicology·2013
    Same author

    The effect of trimethyltin on acetylcholine release in the guinea-pig trachea.

    Environmental toxicology and pharmacology·2011
    Same author

    Enhancement of acetylcholine release by homoanatoxin-a from Oscillatoria formosa.

    Environmental toxicology and pharmacology·2011
    Same author

    Ortho substituted polychlorinated biphenyls elevate intracellular [Ca(2+)] in human granulocytes.

    Environmental toxicology and pharmacology·2011

    Young rats show developing carboxylesterase activity, crucial for detoxification. This enzyme

    Area of Science:

    • Biochemistry
    • Toxicology
    • Developmental Biology

    Background:

    • Carboxylesterase and cholinesterase are key enzymes in xenobiotic metabolism.
    • Understanding their developmental profiles is vital for assessing toxicological risks in young organisms.

    Purpose of the Study:

    • To investigate the ontogeny of carboxylesterase and cholinesterase activity in young rats.
    • To correlate enzyme activity with the toxicity of soman during early development.

    Main Methods:

    • Measurement of carboxylesterase and cholinesterase activity in plasma, liver, and lung homogenates.
    • Enzyme assays using methyl butyrate and 4-nitrophenyl butyrate as substrates.
    • Determination of soman toxicity at different ages (5-31 days old).

    Related Experiment Videos

    Main Results:

    • Cholinesterase activity remained relatively constant across ages and sexes.
    • Plasma carboxylesterase activity significantly increased from 5 to 31 days old.
    • Liver carboxylesterase activity showed a marked increase, while lung activity increased slightly.
    • Soman toxicity was 6-7 times higher in 5-day-old rats compared to 30-day-old rats.
    • Decreased soman toxicity correlated with increased plasma carboxylesterase activity.

    Conclusions:

    • Plasma carboxylesterase activity development is a critical factor in mitigating soman toxicity in young rats.
    • Enzyme ontogeny plays a significant role in age-dependent variations in chemical toxicity.