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Histamine Dynamics During Ingestive Behavior Measured by the Novel Biosensor HisLightG
K L Volcko1, A Gresch2, B Benowitz3
1Department of Psychology, UiT The Arctic University of Norway, Tromsø, Norway.
Journal of Neurochemistry
|July 4, 2025
Summary
Histamine neurotransmitter levels rapidly change during feeding. Enhancing histamine tone reduces food intake, suggesting its role in appetite suppression.
Area of Science:
- Neuroscience
- Neuroendocrinology
- Physiology
Background:
- The neurotransmitter histamine influences food intake regulation.
- Histamine's dynamic changes during feeding episodes are not well understood.
Purpose of the Study:
- To measure real-time histamine release in the hypothalamus during feeding.
- To investigate how hunger, time of day, and caloric content affect histamine fluctuations.
- To explore histamine's role in appetite suppression.
Main Methods:
- Utilized a novel genetically-encoded histamine sensor (HisLightG) and fiber photometry.
- Measured histamine release in the paraventricular nucleus (PVH) and ventromedial hypothalamus (VMH) of mice.
- Administered L-histidine to enhance histamine tone and observed effects on licking behavior.
Main Results:
- Histamine levels exhibited rapid changes in response to eating.
- Histamine responses varied with experimental conditions (e.g., sucralose, light cycle).
- Distinct regional differences in histamine dynamics were observed between the PVH and VMH.
- Increased histamine tone reduced licking behavior across various sucrose concentrations.
Conclusions:
- Histaminergic activity is rapidly modulated during ingestive episodes.
- Understanding histamine release patterns provides insight into appetite suppression mechanisms.
- Histamine plays a significant role in regulating food intake.
