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Updated: Jul 8, 2026

Cell-based Calcium Assay for Medium to High Throughput Screening of TRP Channel Functions using FlexStation 3

Cell-based Calcium Assay for Medium to High Throughput Screening of TRP Channel Functions using FlexStation 3

Published on: August 17, 2011

Intracellular calcium imaging for agonist screening.

Haojie Wang¹, Bo Yang¹, Liangzhu Mo¹

¹Cyrus Tang Medical Institute, Soochow University, Suzhou 215123, Jiangsu, China.

Biophysics Reports

|July 4, 2025

Summary

This study presents a new method for screening drugs that target Calcium Homeostasis Modulator 1 (CALHM1) channels. This research aids in understanding calcium

Keywords:

Calcium homeostasis modulator 1 Calcium imaging Fluo-8 jGCaMP8m

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Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Published on: December 13, 2024

Area of Science:

Physiology
Molecular Biology
Pharmacology

Background:

Calcium ions regulate critical physiological processes including nerve conduction, muscle contraction, cell division, and gene expression via calcium transients and oscillations.
Calcium Homeostasis Modulator 1 (CALHM1) is a newly identified plasma membrane ion channel responsible for calcium influx.
The identification of CALHM1 agonists and antagonists is crucial for advancing research into calcium-related physiological and pathological conditions and for developing targeted therapeutics.

Purpose of the Study:

To establish a comprehensive protocol for detecting real-time calcium dynamics.
To facilitate drug screening and validation for novel CALHM1 modulators.
To provide a guide for investigating calcium-related physiological processes using calcium imaging techniques.

Main Methods:

Utilized genetically encoded calcium indicators in transiently transfected cell lines.
Employed calcium indicator dyes in primary cells for calcium dynamics detection.
Developed a step-by-step approach for drug screening and validation based on calcium imaging.

Main Results:

Successfully demonstrated the detection of real-time calcium dynamics in both cell lines and primary cells.
Outlined a comprehensive methodology for screening and validating potential CALHM1-targeting drugs.
Validated the utility of calcium imaging for studying calcium-related physiological functions.

Conclusions:

The presented protocol offers a valuable tool for researchers studying calcium signaling.
This method enables efficient screening and validation of novel CALHM1 agonists and antagonists.
The findings support the development of new therapeutic strategies for diseases involving calcium dysregulation.