Related Experiment Video
Updated: Sep 17, 2025
11:44
Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
Published on: March 20, 2015
20.6K
Detection of lamivudine using liquid-surface-enhanced Raman spectroscopy
Lebogang Thobakgale1, Lungile Nomcebo Thwala1, Patience Mthunzi-Kufa1,2
1Photonics Centre, Council for Scientific and Industrial Research PO Box 395 Pretoria 0001 South Africa LThobakgale@csir.co.za.
RSC Advances
|July 4, 2025
Summary
This study introduces silver nanoparticles (AgNPs) and liquid-surface enhanced Raman spectroscopy (liquid-SERS) for detecting lamivudine (LAM) in HIV/AIDS treatment. This novel method offers a rapid, cost-effective approach for pharmaceutical quality control at low concentrations.
Area of Science:
- Analytical Chemistry
- Materials Science
- Spectroscopy
Background:
- Antiretroviral drugs like lamivudine (LAM) are crucial for HIV/AIDS treatment.
- Substandard pharmaceutical production necessitates advanced quality control methods.
- Silver nanoparticles (AgNPs) show potential for sensitive chemical detection.
Purpose of the Study:
- To explore the use of AgNPs with liquid-surface enhanced Raman spectroscopy (liquid-SERS) for detecting and quantifying LAM.
- To establish a rapid, cost-effective method for pharmaceutical quality control.
Main Methods:
- AgNPs were synthesized and characterized (size, shape, absorbance, molecular properties).
- LAM samples were spiked with AgNPs and analyzed using liquid-SERS.
- Partial least-square analysis was used to determine linearity, sensitivity, LOD, and LOQ.
Main Results:
- Enhanced sensitivity was observed for LAM detection using specific AgNP and citrate stabilizer spectral bands.
- Linearity (R²) ranged from 0.96-0.98.
- Limit of Detection (LOD) and Limit of Quantification (LOQ) were determined for LAM concentrations.
Conclusions:
- The AgNP-enhanced liquid-SERS method provides a viable approach for detecting LAM at low concentrations.
- This technique is comparable to established methods like UV-vis and HPLC.
- Further research into AgNPs and liquid-SERS can lead to new pharmaceutical quality control strategies.
Related Concept Videos
01:26Raman Spectroscopy Instrumentation: Overview
537
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
537
01:20Raman Spectroscopy: Overview
618
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
618
01:09UV–Vis Spectroscopy: Beer–Lambert Law
4.2K
The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The...
4.2K
01:14UV–Vis Spectrometers
1.5K
The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
1.5K