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Updated: Sep 17, 2025

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors
Published on: December 6, 2018
Effects and mechanisms of low-intensity ultrasound on denitrification system
Jinhao Zhu1, Ran Gao1, Chaoming Zhang2
1School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, People's Republic of China.
Abstract:
The effects of different condition of low-intensity ultrasound on denitrification system was investigated. The effects on the characteristic of denitrifying bacteria were analyzed by measuring the electron transport system activity (ETSA) and extracellular polymeric substances (EPS). Also, the changes in the secondary structure of membrane proteins and lipids were analyzed by Fourier Transform Infrared (FTIR) spectroscopy and explored by second-order derivative fitting using OMNIC and Peakfit software. The results showed that low-intensity ultrasound could improve the mass transfer rate of cells by changing the secondary structure of protein lipid and improving the permeability and fluidity of the membrane. Vacuolization in the cytoplasm, accompanied by cell membrane damage was observed in transmission electron microscope (TEM), which meant ultrasound could disrupt cell membrane structure. ImageJ was employed to analyse TEM images, quantifying the membrane thickness via the ruler tool, it was found that the thickness of cell membrane decreased significantly after ultrasound.
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