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Updated: Jun 29, 2026

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
Published on: February 7, 2018
An esterase-sensitive persulfide/hydrogen sulfide generating fluorogenic probe enhances antioxidant response
Bharat S Choudhary1, Akshi Vashistha2, Ankan Ghosh2
1Department of Chemistry, Indian Institute of Science Education and Research Pune, Pune 411 008, Maharashtra, India. harinath@iiserpune.ac.in.
None:
Hydrogen sulfide (H2S) and sulfane sulfur species such as persulfides and polysulfides protect the cells from oxidative stress and are central to the endogenous antioxidant response. Here, we designed and developed a tool that is cleaved by esterase to generate phenacylthiol, which is an artificial substrate for 3-mercaptopyruvate sulfurtransferase (3-MST) and non-electrophilic lactone as a by-product. The esterase-sensitive persulfide generator enhances H2S and sulfane sulfur and protects chondrocytes from the lethality induced by elevated reactive oxygen species (ROS).
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