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Updated: Jun 29, 2026

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
Published on: February 7, 2018
An esterase-sensitive persulfide/hydrogen sulfide generating fluorogenic probe enhances antioxidant response
Bharat S Choudhary1, Akshi Vashistha2, Ankan Ghosh2
1Department of Chemistry, Indian Institute of Science Education and Research Pune, Pune 411 008, Maharashtra, India. harinath@iiserpune.ac.in.
Hydrogen sulfide (H2S) and related compounds combat oxidative stress. A new tool generates these protective molecules, safeguarding cells from reactive oxygen species (ROS) damage.
Area of Science:
- Biochemistry
- Cell Biology
- Medical Chemistry
Background:
- Hydrogen sulfide (H2S) and sulfane sulfur species are crucial endogenous antioxidants.
- Oxidative stress from reactive oxygen species (ROS) poses a significant threat to cellular health.
Purpose of the Study:
- To develop a novel tool for generating H2S and sulfane sulfur species.
- To investigate the protective effects of this tool against ROS-induced cellular damage.
Main Methods:
- Design and synthesis of an esterase-sensitive persulfide generator.
- Utilizing the tool to release phenacylthiol, an artificial substrate for 3-mercaptopyruvate sulfurtransferase (3-MST).
- Assessing chondrocyte viability under conditions of elevated ROS.
Main Results:
- The developed tool effectively generates H2S and sulfane sulfur species upon esterase cleavage.
- The generated species protect chondrocytes from ROS-induced lethality.
- The tool releases a non-electrophilic lactone as a by-product.
Conclusions:
- The esterase-sensitive persulfide generator is a viable method for enhancing endogenous antioxidant defenses.
- This tool offers a protective strategy against oxidative stress in cellular models.
- The findings have implications for understanding and combating ROS-related cellular damage.
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