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Development of certified reference materials for two acylated anthocyanins.

Guowen Li1, Peiqing Yang2, Xue Wang3

  • 1College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China; Institute of Quality Standards and Testing Technology for Agro-Products of Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-food Safety and Quality, Ministry of Agriculture and Rural Affairs, Beijing 100081, PR China.

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Summary

Two new anthocyanin certified reference materials (CRMs) were developed for accurate anthocyanin analysis. These CRMs ensure reliable quality control and traceability for natural anthocyanin products.

Keywords:
AcylationCharacterizationMass balance methodQuantitative nuclear magnetic resonanceRed cabbageUncertainty

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Area of Science:

  • Analytical Chemistry
  • Food Chemistry
  • Natural Product Analysis

Background:

  • Accurate determination of anthocyanins is essential for quality control of natural products.
  • Certified Reference Materials (CRMs) are crucial for validating analytical methods and ensuring measurement traceability.
  • Existing CRMs for complex anthocyanins are limited, necessitating the development of new standards.

Purpose of the Study:

  • To develop and characterize two novel anthocyanin certified reference materials (CRMs).
  • To establish reliable methods for purity assessment and stability testing of anthocyanin CRMs.
  • To provide tools for accurate anthocyanin quantification in real-world samples.

Main Methods:

  • Development of two specific anthocyanin CRMs: cyanidin 3-O-(2-O-glucosyl, 6-O-sinapoyl)glucoside, 5-O-glucoside trifluoroacetate and cyanidin 3-O-(2-O-glucosyl, 6-O-p-coumaroyl)glucoside, 5-O-glucoside trifluoroacetate.
  • Purity determination using a combination of mass balance (MB) and quantitative nuclear magnetic resonance (qNMR) techniques.
  • Assessment of CRM homogeneity and stability under various storage conditions (4°C and -20°C).

Main Results:

  • Two anthocyanin CRMs were successfully developed with determined purities of 92.9% and 94.7%.
  • Expanded uncertainties for purity were reported as 0.4% and 0.3% (k=2), respectively.
  • The CRMs demonstrated good homogeneity and stability over time, and were effectively applied to real sample analysis.

Conclusions:

  • The developed anthocyanin CRMs provide reliable standards for accurate anthocyanin quantification.
  • These CRMs are valuable for quality control and product traceability in the natural anthocyanin industry.
  • This study offers a practical framework for the future development of diverse anthocyanin CRMs.