Related Experiment Video

Updated: May 1, 2026

Author Spotlight: Advancements in CAR-T Cell Manufacturing and Gene Therapy Production
06:18

Author Spotlight: Advancements in CAR-T Cell Manufacturing and Gene Therapy Production

Published on: August 18, 2023

2.8K

The future belongs to those who believe in multiplex CAR T engineering

Laura Volta1, Chiara F Magnani1

  • 1Department of Medical Oncology and Hematology, University Hospital Zurich and University of Zurich, Zurich, Switzerland.

Cell Stem Cell
|July 4, 2025
PubMed

Insights

Engineered induced pluripotent stem cell-derived CAR T cells overcome toxicity and immune evasion, improving treatment for HER2-positive solid tumors by enhancing tumor targeting and persistence.

Area of Science:

  • Oncology
  • Immunotherapy
  • Stem Cell Biology

Background:

  • CAR T-cell therapy faces challenges in HER2-positive solid tumors due to toxicity and immune evasion.
  • Existing treatments are limited by the tumor microenvironment and antigen escape.

Purpose of the Study:

  • To develop an induced pluripotent stem cell (iPSC)-derived CAR T-cell product for improved solid tumor treatment.
  • To engineer CAR T cells for tumor-selective targeting, enhanced persistence, and overcoming immune evasion.

Main Methods:

  • Generation of iPSC-derived CAR T cells.
  • Engineering for tumor-specific antigen recognition.
  • Enhancement of resistance to the immunosuppressive tumor microenvironment.
  • Optimization for improved cell persistence and trafficking.

Main Results:

  • The engineered iPSC-derived CAR T cells demonstrated tumor-selective targeting.
  • Enhanced resistance to the immunosuppressive tumor microenvironment was observed.
  • Improved cell persistence and trafficking within the tumor were achieved.
  • Mitigation of antigen escape was a key outcome.

Conclusions:

  • iPSC-derived CAR T cells offer a promising platform for overcoming challenges in solid tumor immunotherapy.
  • This engineered cell product shows potential for more effective treatment of HER2-positive solid tumors.

More Related Videos

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research09:12

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research

Published on: June 10, 2025

430
Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates06:10

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

442

Related Concept Videos

Design Example: Automobile Ignition System01:14

Design Example: Automobile Ignition System

The automobile's ignition system plays a vital role by ensuring the timely ignition of the fuel-air mixture in each cylinder. This ignition is facilitated by a spark plug, which is composed of two electrodes separated by an air gap. A spark forms across this air gap when a substantial voltage is generated between the electrodes, leading to the ignition of the fuel.
One can generate a large voltage using a car battery of 12 volts with the help of inductors. Inductors are known for opposing...
700
Design of Transmission Shafts01:16

Design of Transmission Shafts

The design of a transmission shaft is governed by two primary specifications: the power it transmits and its rotational speed. These parameters guide the selection of the shaft's material and cross-sectional dimensions, ensuring that the material's maximum shearing stress remains within the elastic limit while transmitting the desired power at the given speed. The system's power is intrinsically linked to the applied torque. The torque applied to the shaft can be calculated by reconfiguring the...
905
Control Systems: Applications01:25

Control Systems: Applications

Electrical engineering plays a pivotal role in our daily lives, with control systems at the heart of many applications, from home appliances to sophisticated space shuttles. Control systems manage and regulate the behavior of devices and processes, ensuring they function safely, correctly, and efficiently.
In modern vehicles, control systems manage various functions to enhance performance and safety. The steering wheel and accelerator are primary inputs in a car's control system. The...
1.2K
Electro-mechanical Systems01:19

Electro-mechanical Systems

Electromechanical systems are intricate configurations that effectively combine electrical and mechanical elements to achieve a desired outcome. Central to many of these systems is the DC motor, a device that converts electrical energy into mechanical motion, enabling various applications ranging from simple fans to complex robotic mechanisms.
A key component of the DC motor is the armature, a rotating circuit positioned within a magnetic field. As an electric current passes through the...
1.3K
Multi-input and Multi-variable systems01:22

Multi-input and Multi-variable systems

Cruise control systems in cars are designed as multi-input systems to maintain a driver's desired speed while compensating for external disturbances such as changes in terrain. The block diagram for a cruise control system typically includes two main inputs: the desired speed set by the driver and any external disturbances, such as the incline of the road. By adjusting the engine throttle, the system maintains the vehicle's speed as close to the desired value as possible.
In the absence of...
544
Transformers in Distribution System01:27

Transformers in Distribution System

Transformers in distribution systems can be broadly categorized into distribution substation transformers and other distribution transformers. They are crucial for stepping down high transmission voltages to levels suitable for distribution and end-user applications.
Distribution substation transformers come in various ratings and typically use mineral oil for insulation and cooling. To prevent moisture and air from entering the oil, some transformers use an inert gas like nitrogen to fill the...
682