Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Master Transcription Regulators02:23

Master Transcription Regulators

7.1K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.1K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

7.7K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.7K
Negative Regulator Molecules01:23

Negative Regulator Molecules

36.1K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
36.1K
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

3.3K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.3K
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

2.3K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.3K
Lineage Commitment01:21

Lineage Commitment

3.1K
Commitment is the  process whereby stem cells:
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Critical period plasticity enables credit assignment.

bioRxiv : the preprint server for biology·2026
Same author

ROS-pH Dual-Responsive Polydopamine Nanoparticles for Targeted Fasudil Delivery Ameliorate Multiple Pathologies in Diabetic Retinopathy.

International journal of nanomedicine·2026
Same author

A Resampling-Based Framework for Network Structure Learning in High-Dimensional Data.

ArXiv·2026
Same author

Macrophage NRF1 promotes mitochondrial protein turnover via the ubiquitin proteasome system to limit mitochondrial stress and inflammation.

Cell reports·2026
Same author

Sub-phenotypes of pneumonia defined by pulmonary histopathological features.

American journal of respiratory and critical care medicine·2026
Same author

Metabolomic signatures of extreme old age: findings from the New England Centenarian Study.

GeroScience·2026

Related Experiment Video

Updated: Sep 16, 2025

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
09:07

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Published on: June 21, 2016

8.3K

DDR1 regulates RUNX1-CBFβ to control breast stem cell differentiation.

Colin Trepicchio1, Gat Rauner1, Nicole Traugh1

  • 1Department of Developmental, Molecular & Chemical Biology, Tufts University School of Medicine, Boston, MA 02111, USA.

Stem Cell Reports
|July 4, 2025
PubMed
Summary
This summary is machine-generated.

Inhibition of discoidin domain receptor 1 (DDR1) blocks breast stem cell differentiation and tissue development, revealing a key pathway in breast cancer. This DDR1-RUNX1 signaling axis is crucial for normal breast tissue formation.

Keywords:
3D modeladult stem cellsdifferentiationhuman breasthydrogel modelorganogenesisorganoid modelorganoidsregenerationtissue development

More Related Videos

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
06:12

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Published on: March 7, 2022

3.5K
Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells
09:32

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells

Published on: February 27, 2020

9.1K

Related Experiment Videos

Last Updated: Sep 16, 2025

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
09:07

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Published on: June 21, 2016

8.3K
Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
06:12

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Published on: March 7, 2022

3.5K
Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells
09:32

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells

Published on: February 27, 2020

9.1K

Area of Science:

  • Developmental Biology
  • Cancer Biology
  • Stem Cell Research

Background:

  • Epithelial stem cell differentiation and morphogenesis are critical for breast tissue development.
  • Disruptions in these processes are implicated in breast cancer pathogenesis.
  • Understanding the molecular mechanisms governing these processes is essential for identifying therapeutic targets.

Purpose of the Study:

  • To investigate how individual stem cells differentiate and form complex breast tissue using a novel organoid model.
  • To elucidate the role of discoidin domain receptor 1 (DDR1) in breast epithelial stem cell differentiation and morphogenesis.
  • To identify the downstream targets and mechanisms regulated by DDR1 in this process.

Main Methods:

  • Utilized a next-generation single-cell-derived organoid model for studying breast tissue development.
  • Employed inhibition of discoidin domain receptor 1 (DDR1) and disruption of RUNX1 function.
  • Performed mutational analyses on breast cancer patient data to assess the DDR1-RUNX1 signaling axis.

Main Results:

  • Inhibition of DDR1 led to cells being trapped in a bipotent state, halting alveolar morphogenesis and luminal cell expansion.
  • Disruption of RUNX1 function mirrored the DDR1 inhibition phenotype, indicating RUNX1 acts downstream of DDR1.
  • DDR1 regulates RUNX1 activity by influencing its interaction and expression with its cofactor, core binding factor beta (CBFβ).
  • Frequent alterations and co-occurring mutations in the DDR1-RUNX1 signaling axis were observed in breast cancer patients.

Conclusions:

  • The DDR1-RUNX1 signaling pathway is a central regulator of breast epithelial stem cell differentiation and morphogenesis.
  • Dysregulation of this pathway, particularly through mutations, is fundamentally linked to breast cancer development.
  • These findings highlight the DDR1-RUNX1 axis as a potential therapeutic target for breast cancer.