Polydopamine-modified collagen membrane loading with platelet-rich plasma for enhancing diabetic wound healing

Platelet-rich plasma (PRP), a reservoir of growth factors, is instrumental in the repair and regeneration of damaged tissues, orchestrating wound healing at all stages. However, PRP's rapid degradation and instability at the wound site, prone to displacement and degradation, limit its efficacy. Collagen, the most abundant protein in the human body, boasts exceptional biocompatibility, biological activity, and minimal immunogenicity. Polydopamine (PDA)-coated materials have been employed for sustained drug release, leveraging the catechol, amine, and imine functional groups on their surface for covalent bonding with other molecules. This study presents the fabrication of a PDA-modified collagen membrane (PDA-CM) loaded with PRP (PDA-CM@PRP) to achieve a sustained release of PRP. Our results showed that PDA-CM@PRP significantly improved proliferation, migration, delayed cellular senescence and reduced oxidative stress in human dermal fibroblasts (HDFs)in vitro.In vivoexperiments demonstrated accelerated diabetic wound healing with enhanced granulation tissue formation, cell proliferation, and neovascularization. Transcriptome sequencing analysis revealed that PDA-CM@PRP activated HDFs proliferation through upregulation of the cell cycle and DNA replication pathways. This study presents a novel strategy for sustained PRP release, offering a promising therapeutic approach for diabetic wounds and other chronic wound types.