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Updated: Sep 16, 2025
Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies
Published on: January 23, 2019
Improved methanol utilization of Komagataella phaffii by rebuilding metabolic network based on new alcohol-aldehyde
Kang Li1, Xiuxia Liu1, Chunli Liu1
1School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China; National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China; Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China.
Abstract:
Bioconversion based on methanol holds promise for advancing global carbon neutrality and mitigating the global food crisis. However, the limited efficiency of methanol utilization hampers methanol-based biomanufacturing in microorganisms. This study focuses on enhancing methanol utilization efficiency in Komagataella phaffii through the implementation of a novel alcohol-aldehyde conversion system based on the Adh900 protein. Results indicate a notable 14 % increase in biomass compared to the wild-type strain. Furthermore, analysis of the amino acid metabolism pathway underscores the significance of histidine in facilitating the methanol utilization pathway (MUTP). By combination of adding 120 mg/L histidine to the medium and optimizing promoter strategies, the dry cell weight (DCW) was up to 4.33 g DCW/L, which was increased by 63 %. The combination of these strategies has important research significance for methanol-based biological metabolism, and will further promote the microbial application of methanol. These findings are poised to advance the development of methanol-nutritive cell factories, thereby propelling the evolution of methanol biomanufacturing technology.
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