Detection of Matrix Metalloproteinase 2 by a Polypeptide Cleavable Split-Type Sensor Based on Atomically Precise
1Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P. R. China.
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In this study, a split-type electrochemiluminescence (ECL) sensor based on polypeptide cleavage was proposed for the sensitive detection of matrix metalloproteinase 2 (MMP 2). The bimetallic nanoclusters were protected by mercaptosuccinic acid (MSA), named Au3Ag5(MSA)3, serving as a signal probe. Owing to the synergistic effect of the bimetal composition, the clusters exhibited enhanced ECL properties. Polypeptide chains (PLGVR) that can be specifically cleaved by MMP 2 were selected from the MMP 2 domain. Additionally, the antifouling segment (PPEKEK) and the binding segment (CCC) were incorporated into the polypeptide chain design. In the presence of MMP 2, a specific cleavage site on the polypeptide was targeted, resulting in the detachment of Au3Ag5(MSA)3 and subsequent generation of an ECL signal. Compared to conventional detection methods, this approach exhibited enhanced sensitivity and improved stability. This method provides a valuable reference for detecting members of the matrix metalloproteinase family and holds promising prospects for future development.
