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Related Experiment Videos

An electrophoretic device concentrating charged macromolecules to a predetermined final solution volume.

B T Stokke, A Mikkelsen, A Elgsaeter

    Analytical Biochemistry
    |August 1, 1985
    PubMed
    Summary

    A new apparatus achieves near 100% yield for electrophoretic concentration of macromolecules. This device minimizes aggregation and denaturation, offering precise control over final volume and concentration.

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    Area of Science:

    • Biochemistry
    • Biophysical Chemistry
    • Analytical Chemistry

    Background:

    • Electrophoretic concentration is crucial for macromolecule analysis.
    • Conventional methods face challenges with aggregation, adsorption, and denaturation.
    • Precise control over final volume and concentration remains difficult.

    Purpose of the Study:

    • To develop an apparatus for electrophoretic concentration of charged macromolecules.
    • To achieve a predetermined final solution volume and concentration with high yield.
    • To minimize macromolecule degradation during concentration.

    Main Methods:

    • Development of a novel apparatus for electrophoretic concentration.
    • Implementation of one-stage and two-stage concentration processes.

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  • Characterization of concentration yield, final volume, and concentration independence from electrophoresis time.

    • Main Results:

    • Near 100% yield achieved for macromolecule concentration.
    • Final volume and concentration are largely independent of electrophoresis time.
    • Low electric field strength at the boundary reduces aggregation, adsorption, and denaturation.
    • One-stage apparatus yields 10-fold concentration; two-stage yields 50-fold concentration.
    • Apparatus effective for initial volumes of 20-50 ml.

    Conclusions:

    • The developed apparatus offers efficient and controlled electrophoretic concentration of macromolecules.
    • The design minimizes undesirable side reactions, preserving macromolecule integrity.
    • This technology provides a significant advancement over conventional electrophoretic concentrators.