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Updated: Sep 16, 2025

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
Published on: February 14, 2022
Raman spectroscopy of deuterium-labeled macromolecules: Emerging strategies in biomedical research
Zefeng Zheng1, Ying Cui2, Boyou Zhang3
1Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children's Regional Medical Center, Hangzhou 310052, China; Zhejiang Key Laboratory of Neonatal Diseases, Hangzhou 310052, China.
Abstract:
When macromolecules are exposed to deuterium-rich environments, hydrogen atoms on them undergo hydrogen‑deuterium exchange, which induces a characteristic Raman spectral redshift, providing a robust signature for probing intracellular microenvironments. This review is a comprehensively survey of the diverse applications of Raman spectroscopy of deuterium-labeled macromolecules in biomedicine over recent years. We summarize its utility in bacterial resistance and metabolism investigations, and discuss applications spanning cellular to whole-animal levels.
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