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Updated: May 5, 2026
Preparation and Characterization of Individual and Multi-drug Loaded Physically Entrapped Polymeric Micelles
Published on: August 28, 2015
Encapsulation of Plicosepalus curviflorus-derived polyphenols in chitosan-alginate microvehicles for potent
Wesam Abd El-Fattah1, Ahlem Guesmi1, Naoufel Ben Hamadi1
1Department of Chemistry, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), P.O. Box 5701, Riyadh 11432, Saudi Arabia.
Abstract:
The main objective of this study was to apply response surface methodology (RSM) to optimize the microencapsulation of therapeutic P. curviflorus-derived total polyphenols (TPP) using chitosan-sodium alginate microcapsules (MCs), targeting the fabrication of TPP-loaded MCs (TPP@MCs) for effective colorectal adenocarcinoma therapy. Using a Box-Behnken design, we systematically varied the microcapsule composition, TPP loading ratio, and crosslinking or sonication time to maximize the encapsulation efficiency (EE). TPP-loaded MCs (TPP@MCs) were formulated using an emulsification-ionotropic gelation approach. The structural and morphological characteristics of the TPP@MCs were investigated using spectroscopic and microscopic methods. The EE of the optimized microcapsules was quantified to be 90.5607 %. The TPP release curve displayed a distinct biphasic pattern influenced by pH, initiating with a rapid release and transitioning into a prolonged and controlled-release phase. MTT results indicated that TPP@MCs had much higher selective cytotoxicity against SW620 cells than their precursors, suggesting that encapsulating TPP in MCs improves their anti-breast cancer activity.
