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Updated: Sep 16, 2025

In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
Published on: July 25, 2019
Regulation, functions and therapeutic strategies of H2AK119ub1
Yu Zhang1, Kaiwen Xu1, Keyi Zhang1
1School of Pharmacy and Science, Institute of Clinical Pharmacology, Key Laboratory of Anti-inflammatory and Immune Medicine, Ministry of Education, Anhui Medical University, Hefei 230032, China.
Abstract:
Histone H2A monoubiquitination at lysine 119 (H2AK119ub1) is a central epigenetic regulator that governs transcriptional control, chromatin architecture remodeling, and lineage commitment. The precise homeostatic balance of H2AK119ub1 is maintained through antagonistic coordination between polycomb repressive complex 1 and specialized deubiquitinases, forming a precise equilibrium that is critical for the regulation of gene expression. Dysregulation of H2AK119ub1 has been mechanistically linked to multiple human pathologies, indicating that its regulatory axis is a promising therapeutic target. This review provides a comprehensive analysis of the molecular mechanisms underlying H2AK119ub1 deposition/removal, its causal relationships with disease progression, and current pharmacological strategies targeting this pathway. This study offers novel perspectives on H2AK119ub1-mediated epigenetic regulation and proposes a framework for developing targeted therapeutic interventions.
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