Jove
Visualize
Contact Us

Related Concept Videos

Histone Modification02:32

Histone Modification

3.6K
3.6K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

8.5K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
8.5K
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

4.5K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Uniform zinc oxide nanowire arrays grown on nonepitaxial surface with general orientation control.

Nano letters·2013
Same author

[American head and neck surgery progress of in 2012].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2013
Same author

A compact thermo-optical multimode-interference silicon-based 1 × 4 nano-photonic switch.

Optics express·2013
Same author

Experimental demonstration of 110-Gb/s unsynchronized band-multiplexed superchannel coherent optical OFDM/OQAM system.

Optics express·2013
Same author

Potentially functional variants of p14ARF are associated with HPV-positive oropharyngeal cancer patients and survival after definitive chemoradiotherapy.

Carcinogenesis·2013
Same author

Enhanced molecular transport in hierarchical silicalite-1.

Langmuir : the ACS journal of surfaces and colloids·2013
Same journal

Tunable self-assembling cellular microarray for single-neutrophil vital and suicidal extracellular traps.

Lab on a chip·2026
Same journal

Precise programmable tumor cell subpopulation sorting <i>via</i> an electromagnetic microfluidic platform.

Lab on a chip·2026
Same journal

Bridging dimensions: combining one- and two-photon 3D printing for microfluidic device fabrication.

Lab on a chip·2026
Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026
Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026
Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Sep 16, 2025

Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF
05:52

Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF

Published on: January 12, 2024

1.2K

Lossless Altered Histone Modification Analysis System (LAHMAS).

Zachary J Kauffman1, Kevin Koesser2, Kyle T Helzer3

  • 1Department of Medicine, University of Wisconsin-Madison, Madison, WI, USA. jsperger@medicine.wisc.edu.

Lab on a Chip
|July 7, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a novel microfluidic system for miniaturized biological assays, improving sensitivity and reducing sample loss. The Lossless Altered Histone Modification Analysis System (LAHMAS) enables accurate chromatin profiling from rare cells.

More Related Videos

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
11:02

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis

Published on: May 17, 2016

29.5K
The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
24:02

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

18.3K

Related Experiment Videos

Last Updated: Sep 16, 2025

Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF
05:52

Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF

Published on: January 12, 2024

1.2K
Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
11:02

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis

Published on: May 17, 2016

29.5K
The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
24:02

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

18.3K

Area of Science:

  • Biotechnology
  • Microfluidics
  • Molecular Biology

Background:

  • Miniaturizing biological assays with microfluidics offers enhanced sensitivity and throughput but faces challenges like evaporation and analyte loss.
  • Exclusive Liquid Repellency (ELR) addresses these issues by using an oil phase to protect aqueous volumes and prevent surface binding.
  • CUT&Tag is a complex multistep assay that can benefit from miniaturization for improved efficiency and reduced sample requirements.

Purpose of the Study:

  • To develop a novel microfluidic platform for miniaturizing the CUT&Tag assay.
  • To leverage Exclusive Liquid Repellency (ELR) and Exclusion-based Sample Preparation (ESP) for lossless liquid handling in microfluidic systems.
  • To create a system capable of accurate chromatin profiling from low input and rare cell samples.

Main Methods:

  • Developed a microfluidic platform using PDMS-silane treated glass surfaces immersed in silicone oil.
  • Integrated Exclusive Liquid Repellency (ELR) and Exclusion-based Sample Preparation (ESP) principles.
  • Utilized the platform for miniaturized CUT&Tag assays, named the Lossless Altered Histone Modification Analysis System (LAHMAS).

Main Results:

  • The LAHMAS platform demonstrated lossless liquid handling and prevented sample evaporation.
  • Effective processing of cell inputs as low as 100 cells was achieved.
  • Higher specificity compared to macroscale CUT&Tag assays was observed, enabling accurate chromatin profiling.

Conclusions:

  • The novel microfluidic platform effectively miniaturizes the CUT&Tag assay, overcoming common challenges.
  • LAHMAS facilitates accurate chromatin profiling of low input and rare cell samples with enhanced specificity.
  • This technology holds potential for advancing biological assays in various research applications.