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Related Concept Videos

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Mesenchymal Stem Cells

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Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
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When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
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Related Experiment Video

Updated: Sep 16, 2025

Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model
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Soluble CCR2-Expressing Mesenchymal Stem Cells Inhibit Osteoarthritis Development and Progression.

Hyun Sik Na1, Seon-Young Lee1, Dong Hwan Lee2

  • 1Lab of Translational ImmunoMedicine, Catholic Research Institute of Medical Science, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea.

Immune Network
|July 7, 2025
PubMed
Summary

Soluble C-C chemokine receptor type 2 (sCCR2)-expressing stem cells (sCEMs) offer superior osteoarthritis treatment by inhibiting MCP-1, reducing pain, and protecting cartilage. This gene therapy shows significant therapeutic potential for OA.

Keywords:
CCL2Cell therapyMesenchymal stem cellsOsteoarthritissCCR2

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Flow Cytometry Analysis of Immune Cell Subsets within the Murine Spleen, Bone Marrow, Lymph Nodes and Synovial Tissue in an Osteoarthritis Model
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Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Orthopedics

Background:

  • Osteoarthritis (OA) is a degenerative joint disease often treated with pain relievers or stem cells.
  • Previous research indicated therapeutic effects of soluble C-C chemokine receptor type 2 (sCCR2) gene therapy for OA.
  • Mesenchymal stem cells (MSCs) are explored for cartilage repair, but their efficacy can be enhanced.

Purpose of the Study:

  • To evaluate if sCCR2-expressing MSCs (sCEMs) demonstrate superior efficacy over MSCs alone in an OA model.
  • To investigate the therapeutic mechanisms of sCEMs in inhibiting OA progression.
  • To assess the potential of sCEMs as an effective OA treatment.

Main Methods:

  • An osteoarthritis (OA) rat model was established using monosodium iodoacetate induction.
  • Soluble C-C chemokine receptor type 2 (sCCR2) was transfected into chondrocytes and MSCs to create sCEMs.
  • In vitro and in vivo experiments were conducted, including analysis in MCP-1 conditional knockout mice.

Main Results:

  • MCP-1 was found to inhibit chondrogenesis, while sCEMs demonstrated an improvement in chondrogenesis.
  • sCEM treatment significantly suppressed OA disease development in the rat model.
  • sCEM injections reduced pain, inflammation, and cartilage damage in OA rats compared to MOCK-MSCs.

Conclusions:

  • sCEMs effectively inhibit MCP-1, leading to reduced pain and cartilage damage in OA.
  • The findings suggest that sCEMs possess chondroprotective properties.
  • Inhibiting MCP-1/CCR2 signaling presents a promising therapeutic strategy for osteoarthritis.