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Related Concept Videos

Acute Kidney Injury II: Pathophysiology01:29

Acute Kidney Injury II: Pathophysiology

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Acute kidney injury (AKI) causes are categorized into three primary categories based on the location of the injury: prerenal, intrarenal (or intrinsic), and postrenal causes. This classification guides clinical management and illustrates how different pathways can impair kidney function.Etiology and Pathophysiology of Acute Kidney Injury1. Prerenal causesEtiology: Prerenal Acute Kidney Injury, the most common type, occurs when reduced blood flow to the kidneys decreases filtration capacity...
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Acute Kidney Injury I: Introduction01:22

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Introduction:Acute Kidney Injury (AKI) describes a swift decrease in kidney function occurring over hours to days, characterized by the kidneys' failure to remove waste products from the bloodstream. This leads to dangerous complications like metabolic acidosis, fluid overload, and electrolyte imbalances, such as hyperkalemia, which can cause life-threatening arrhythmias. AKI is common in both hospital and outpatient settings, often triggered by dehydration, sepsis, or exposure to nephrotoxic...
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Acute Kidney Injury IV: Diagnostic Studies and Prevention01:30

Acute Kidney Injury IV: Diagnostic Studies and Prevention

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Accurate diagnosis and effective prevention are critical in managing Acute Kidney Injury (AKI), which is linked to high mortality rates ranging from 10% to 80%. Timely recognition of at-risk patients and careful monitoring can significantly reduce the likelihood of kidney damage.Diagnostic Assessments:The diagnostic process starts with a comprehensive medical history to identify prerenal, intrarenal, and postrenal causes.Prerenal causes, such as dehydration, hypotension, or blood loss, should...
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Acute Kidney Injury III: Clinical Manifestations01:29

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Acute Kidney Injury (AKI) progresses through distinct clinical phases: the oliguric, diuretic, and recovery phases, each marked by unique manifestations and challenges.Oliguric Phase:The oliguric phase is the initial stage of AKI, typically lasting 10 to 14 days. This phase is marked by a significant reduction in urine output, usually less than 400 mL per day, indicating decreased kidney function. Fluid retention is a prominent feature, leading to symptoms such as edema, hypertension, and...
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Acute Kidney Injury V: Interprofessional Care01:20

Acute Kidney Injury V: Interprofessional Care

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Acute Kidney Injury (AKI) requires a collaborative healthcare approach to restore renal function and prevent complications. Essential management strategies involve monitoring fluid and electrolyte balance, adjusting medications, initiating dialysis when necessary, and providing nutritional support.Fluid and Electrolyte ManagementFluid Monitoring: Regularly monitoring body weight, central venous pressure, and urine output helps detect fluid imbalances early. Patient intake and output are...
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Acute Kidney Injury VI: Nursing Management01:22

Acute Kidney Injury VI: Nursing Management

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Acute Kidney Injury (AKI) results in an inability to maintain fluid, electrolyte, and acid-base balance. Effective nursing management is critical in improving patient outcomes and includes comprehensive patient assessment and targeted interventions.Comprehensive Patient AssessmentA detailed history collection is essential, focusing on any recent infections, nephrotoxic medication use, or chronic conditions such as hypertension and diabetes that may contribute to AKI. During the physical...
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Unveiling PANoptosis in Acute Kidney Injury: An Integrative Multi-Dimensional Approach to Identify Key Biomarkers.

Ning Wang1, Lina Zhang1,2, Ziyu Xu1

  • 1Department of Nephrology, Zhengzhou University People's Hospital, Henan Provincial People's Hospital, Zhengzhou, People's Republic of China.

Journal of Inflammation Research
|July 7, 2025
PubMed
Summary

This study identifies five key genes (EGR1, CEBPD, HSPA1A, HSPA1B, RHOB) involved in PANoptosis, a programmed cell death process, and their significant elevation in acute kidney injury (AKI). These findings offer potential biomarkers for AKI diagnosis and treatment.

Keywords:
PANoptosisacute kidney injurybiomarkersmachine learningsingle-cell RNA sequencing

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Area of Science:

  • Molecular Biology
  • Immunology
  • Genetics

Background:

  • Programmed cell death and inflammation are central to acute kidney injury (AKI) progression.
  • PANoptosis, a regulated cell death form, integrates apoptosis, pyroptosis, and necroptosis, but its role in AKI is not well understood.

Purpose of the Study:

  • To investigate the role of PANoptosis in AKI.
  • To identify potential biomarkers for AKI associated with PANoptosis.

Main Methods:

  • Single-cell RNA sequencing (scRNA-seq) and bulk transcriptomic data were used to identify differentially expressed genes (DEGs) related to apoptosis, pyroptosis, and necroptosis in AKI.
  • Machine learning algorithms identified key PANoptosis-associated genes.
  • Receiver operating characteristic (ROC) curve analysis assessed diagnostic potential.
  • Western blot and immunohistochemistry validated findings in AKI animal models.

Main Results:

  • PANoptosis levels were significantly elevated in AKI, with cell-type-specific heterogeneity.
  • Five key upregulated genes (EGR1, CEBPD, HSPA1A, HSPA1B, RHOB) were identified as potential PANoptosis biomarkers in AKI.
  • These genes demonstrated high diagnostic accuracy (AUCs ranging from 0.920 to 0.981) and were validated in AKI models.

Conclusions:

  • This study identifies five key PANoptosis-related genes in AKI using integrated transcriptomic data and machine learning.
  • These genes serve as potential biomarkers for AKI diagnosis and therapeutic targeting.
  • The findings provide novel insights into the role of PANoptosis in AKI pathogenesis.