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Propagation of Homalodisca coagulata virus-01 via Homalodisca vitripennis Cell Culture
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[Grapevine fanleaf virus biology].

Corinne Schmitt-Keichinger1, Marc Fuchs2

  • 1Université de Strasbourg, Inrae, UMR A1131 Santé de la vigne et qualité du vin, Colmar, France.

Virologie (Montrouge, France)
|July 7, 2025
PubMed
Summary
This summary is machine-generated.

Grapevine fanleaf degeneration disease, caused by Grapevine fanleaf virus (GFLV), impacts vineyards globally. While much is known about GFLV, effective vineyard management strategies remain limited, necessitating further research into its infection cycle.

Keywords:
Xiphinema indexbiologygrapevinegrapevine fanleaf virushypersensitive response

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Area of Science:

  • Plant Pathology
  • Virology
  • Molecular Biology

Background:

  • Fanleaf degeneration disease, historically known as "ortiage," is a significant global grapevine disease.
  • Grapevine fanleaf virus (GFLV) is the primary etiological agent, transmitted by the nematode vector Xiphinema index.
  • GFLV was identified in 1960, with its vector Xiphinema index identified as the exclusive transmitter.

Purpose of the Study:

  • To review the current understanding of Grapevine fanleaf virus (GFLV) biology and infection mechanisms.
  • To highlight the progress in characterizing the viral genome, proteins, and virion structure.
  • To identify challenges in GFLV management and suggest future research directions.

Main Methods:

  • Characterization of the bipartite viral genome expression and protein functions.
  • Determination of virion structure via crystallography.
  • Identification of viral determinants for symptom expression, RNA silencing suppression, and metabolic pathway dysregulation.

Main Results:

  • Most viral proteins' functions have been assigned, and viral RNAs were found to be monouridylated.
  • Transmission determinants were mapped to the coat protein, and viral factors influencing leaf and root symptoms were identified.
  • Three suppressors of RNA silencing and dysregulated metabolic pathways in infected plants were identified.

Conclusions:

  • Significant advancements have been made in understanding GFLV molecular biology and infection processes.
  • Despite extensive research, effective GFLV management strategies in vineyards are still limited.
  • Future research focusing on the GFLV infection cycle is crucial for developing transformative management solutions.