MOF-Integrated Single-Enzymatic Colorimetric Paper-Based Biosensor for Point-of-Care Testing of Organophosphorus...

Area of Science:

• Analytical Chemistry
• Environmental Science
• Biotechnology

Background:

• Organophosphorus pesticides (OPs) pose environmental and health risks due to acetylcholinesterase (AChE) inhibition.
• Conventional point-of-care testing (POCT) for OPs often involves complex multi-enzyme systems.
• There is a need for sensitive, selective, and stable POCT methods for OPs.

Purpose of the Study:

• To develop a simplified, highly sensitive, and stable paper chip for POCT of OPs.
• To integrate AChE and a colorimetric reagent into a MOF-based paper chip.
• To create a portable and instrument-free detection platform for OPs.

Main Methods:

• Developed a MOF-based paper chip (AA@ZIF@Paper) integrating AChE and a colorimetric reagent.
• Utilized a single enzymatic reaction with peroxidase-like acetylcholine (ATCI) as the substrate.
• Incorporated a 3D-printed device and smartphone application for enhanced usability.

Main Results:

• Achieved a linear detection range of 0.5-50 μg/L for dichlorvos within 20 min.
• Obtained a low limit of detection (LOD) of 0.29 μg/L.
• Demonstrated improved stability (pH, temperature, storage) due to MOF encapsulation.

Conclusions:

• The MOF-integrated single-enzymatic paper chip provides a promising platform for instrument-free, portable POCT of OPs.
• This method simplifies detection, enhances stability, and improves convenience for food safety and environmental monitoring.
• The developed system offers a sensitive and selective approach for rapid OP analysis.