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Related Experiment Video

Updated: May 16, 2026

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids
10:40

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

Published on: June 5, 2020

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Whole-mount Retinal Organoid Visualization with Cellular Resolution.

Marina Cunquero1, Helena Isla-Magrané2, Maria Marsal1

  • 1ICFO-Institut de Ciències Fotòniques, The Barcelona Institute of Science and Technology.

Journal of Visualized Experiments : Jove
|July 7, 2025
PubMed
Summary
This summary is machine-generated.

Human retinal organoids, derived from stem cells, offer personalized medicine insights. New 3D imaging techniques visualize retinal cell structures, advancing disease research and therapeutic strategies.

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Whole-mount Retinal Organoid Visualization with Cellular Resolution
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Related Experiment Videos

Last Updated: May 16, 2026

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Published on: June 5, 2020

16.7K
Retinal Organoid Induction System for Derivation of 3D Retinal Tissues from Human Pluripotent Stem Cells
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Whole-mount Retinal Organoid Visualization with Cellular Resolution
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Whole-mount Retinal Organoid Visualization with Cellular Resolution

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613

Area of Science:

  • Biomedical Engineering
  • Developmental Biology
  • Stem Cell Biology

Background:

  • Human retinal organoids (HROs) from induced pluripotent stem cells (iPSCs) mimic retinal structure and function.
  • HROs are valuable for studying retinal development, diseases, and personalized medicine.
  • Characterizing complex HROs is challenging due to their 3D architecture and cellular density.

Purpose of the Study:

  • To develop and apply advanced 3D imaging techniques for comprehensive HRO characterization.
  • To visualize the spatial organization of retinal neurons within whole-mount HROs.
  • To enhance understanding of HRO maturation and cellular distribution.

Main Methods:

  • Combined optical clearing with immunolabeling for whole-mount HRO visualization.
  • Utilized confocal microscopy compatible with various numerical apertures for full-volume imaging.
  • Applied a clearing method that preserves 3D context for high-resolution imaging of specific regions.

Main Results:

  • Successfully visualized the 3D morphology and distribution of key retinal neurons (photoreceptors, bipolar cells, ganglion cells).
  • Demonstrated a method for high-resolution, full-volume imaging of complex retinal organoid structures.
  • Provided insights into the spatial organization of neuronal pathways within HROs.

Conclusions:

  • Optical clearing combined with immunolabeling and confocal microscopy is effective for 3D HRO analysis.
  • This technique advances the study of retinal development and disease modeling using organoids.
  • The findings facilitate a deeper understanding of cellular organization in retinal organoids for therapeutic applications.