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Updated: Sep 16, 2025

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
Published on: May 28, 2012
Structural characteristics and immunomodulatory activity of an exopolysaccharide from Antarctic Pseudoalteromonas sp.
Dezhi Wang1, Han Li1, Yu Zhao1
1National Glycoengineering Research Center, School of Life Science, Shandong University, Qingdao, 266237, China.
Abstract:
Natural polysaccharides from microorganisms have various biological activities. However, those from psychrophiles with immunomodulatory properties have rarely been found. In this study, an immunoactive exopolysaccharide was extracted from Antarctic Pseudoalteromonas sp. LP6-12-2 (EPS1-1), which isolated using DEAE-Sepharose Fast Flow, Sephadex G-75, and Sephadex G-100. EPS1-1, with a molecular weight 23.47 kDa, was composed of mannose, glucose, galactose, glucosamine, and galactosamine in the ratio of 83.1:7.2:4.3:4.2:1.2. Furthermore, EPS1-1 was found to have a main chain composed of α-D-Manp (1→2) and side chains composed of α-D-Manp (1→6) by FT-IR, methylation, and NMR analyses. Besides, through the Congo red test and I2-KI reaction analyses, it was revealed that EPS1-1 lacked triple-helix structure and had more branches. Additionally, EPS1-1 could significantly advance the proliferation, phagocytosis, acid phosphatase activity, and cytokines production of macrophage cells. Data suggested EPS1-1 demonstrated potential as an immunomodulator for functional food or pharmaceutical development.
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