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Identification of disulfidptosis-related prognostic biomarkers associated with CD4 + and CD8 + T cells infiltration for sarcoma by integrating bioinformatic analysis and experimental validation

  • 0Center of Hepatobiliary and Pancreatic Surgery, Medical Center of Digestive Disease, Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University, Zhuzhou, 412000, China.
Discover Oncology +

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July 8, 2025

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July 8, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

Disulfidptosis, a novel cell death, involves specific genes like CCDC69 and HMGB3. These genes can predict sarcoma prognosis and immune cell infiltration, offering potential diagnostic and therapeutic targets.

Area Of Science

  • Oncology
  • Cell Biology
  • Immunology

Background

  • Disulfidptosis is a newly identified cell death pathway induced by disulfide stress.
  • The prognostic significance of disulfidptosis-related genes (DRGs) in sarcoma remains largely unknown.
  • Understanding DRG patterns is crucial for advancing sarcoma diagnosis and treatment.

Purpose Of The Study

  • To investigate the impact of DRG patterns on sarcoma patient prognosis.
  • To identify novel prognostic biomarkers for sarcoma subtypes.
  • To explore the relationship between DRGs, immune infiltration, and sarcoma progression.

Main Methods

  • Bioinformatic analysis to identify prognostic DRGs.
  • Development and validation of a prognosis prediction model using Kaplan-Meier (KM) and Area Under the Curve (AUC) analyses.
  • Analysis of gene expression and immune cell infiltration using immunohistochemistry (IHC).

Main Results

  • CCDC69 and HMGB3 were identified as key prognostic DRGs in sarcoma.
  • The developed model demonstrated high accuracy in predicting sarcoma prognosis.
  • Differential expression of CCDC69 and HMGB3 correlated with patient survival and immune cell infiltration (CD4+, CD8+ T cells).
  • HMGB3 expression was significantly higher in sarcoma tissues compared to normal tissues.

Conclusions

  • CCDC69 and HMGB3 serve as valuable prognostic biomarkers for sarcoma.
  • These genes are linked to immune cell infiltration, suggesting a role in the tumor microenvironment.
  • CCDC69 and HMGB3 represent potential targets for sarcoma immunotherapy and clinical diagnosis.

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