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Related Concept Videos

  1. Home
  3. Research Domains
  5. Agricultural, Veterinary And Food Sciences
  7. Agricultural Biotechnology
  9. Agricultural Biotechnology Diagnostics (incl. Biosensors)
  11. Dual-enzyme Cascade Colorimetric Biosensor For Safe, Rapid On-site Detection And Detoxification Of Ochratoxins.
  1. Home
  3. Research Domains
  5. Agricultural, Veterinary And Food Sciences
  7. Agricultural Biotechnology
  9. Agricultural Biotechnology Diagnostics (incl. Biosensors)
  11. Dual-enzyme Cascade Colorimetric Biosensor For Safe, Rapid On-site Detection And Detoxification Of Ochratoxins.

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Dual-enzyme cascade colorimetric biosensor for safe, rapid on-site detection and detoxification of ochratoxins.

Xiao Liang1, Zhuobing Zhang2, Yilei Du3

  • 1Key Laboratory of Engineering Biology for Low-carbon Manufacturing, National Engineering Research Center for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China; National Technology Innovation Center of Synthetic Biology, Tianjin, 300308, China.

Biosensors & Bioelectronics
|July 8, 2025

View abstract on PubMed

Summary
This summary is machine-generated.

A novel colorimetric biosensor simultaneously detects and removes ochratoxins (OTA/OTB) in food. This dual-enzyme system offers rapid, on-site monitoring and detoxification, enhancing food safety.

Keywords:
Accurate detectionDetoxificationEnzyme-based colorimetric biosensorOchratoxins

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Area of Science:

  • Food Science
  • Biotechnology
  • Analytical Chemistry

Background:

  • Ochratoxins (OTA/OTB) are toxic mycotoxins found in food and feed, posing significant human health risks.
  • Current detection methods often lack integrated detoxification capabilities, creating a need for advanced monitoring tools, especially in developing regions.

Purpose of the Study:

  • To develop a safe, convenient, and rapid on-site biosensor for simultaneous detection and removal of Ochratoxin A (OTA) and Ochratoxin B (OTB).
  • To integrate ochratoxin detoxification with a colorimetric detection system for real-time food safety assessment.

Main Methods:

  • Constructed a dual-enzyme cascade biosensor using an ochratoxin-detoxifying enzyme and phenylalanine dehydrogenase.
  • Utilized 1-methoxy-5-methylphenazinium methyl sulfate (MPMS) as a signal reporter for colorimetric signal generation.
Safe and portable
  • Validated the biosensor's performance using spiked Chinese liquor samples and analyzed results with a microplate reader and smartphone.

    • Main Results:

    • The biosensor successfully detoxified and detected OTA/OTB, converting ochratoxin concentration into a visual red-to-green color shift.
    • Achieved effective elimination and detection of OTA/OTB at 5 μg/L in Chinese liquor within 1 hour.
    • Demonstrated quantitative analysis of OTA/OTB in the range of 0-50 μg/mL via microplate reader or smartphone.

    Conclusions:

    • The developed dual-enzyme cascade colorimetric biosensor provides a safe, portable, rapid, and accurate method for OTA/OTB detection and elimination.
    • This innovative tool significantly contributes to on-site food and environmental safety monitoring.
    • The biosensor's dual function of detoxification and detection addresses limitations of existing ochratoxin monitoring systems.