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Updated: Sep 16, 2025

Protocols for Robust Herbicide Resistance Testing in Different Weed Species
Published on: July 2, 2015
Reduced sensitivity of winter Wheat (Triticum aestivum) to triketone herbicides is a partially dominant, polygenic
Susee Sudhakar1, Mithila Jugulam2
1Department of Crop, Soil and Environmental Sciences, University of Arkansas, Fayetteville, Arkansas, USA.
Background:
Wheat production is challenged by weed competition and herbicide-resistant weeds, necessitating alternative herbicides with distinct modes of action. Mesotrione and tembotrione, typically used in corn, are not registered for wheat. Prior research identified wheat genotypes WW-1 and WW-2 as least sensitive and WW-24 as most sensitive to these herbicides, likely due to cytochrome P450-mediated metabolism. To examine the inheritance of this sensitivity, we crossed these genotypes and analyzed F1 and F2 progenies.
Results:
Dose-response experiments on F1 progenies revealed intermediate sensitivity to both herbicides, indicating partial dominance. F2 progenies treated with mesotrione (630 g ai h-1) or tembotrione (552 g ai h-1) did not display Mendelian single-gene inheritance patterns. This suggests that the reduced sensitivity in WW-1 and WW-2 is a polygenic trait associated with P450-mediated herbicide metabolism.
Conclusion:
This study reveals genetic mechanisms underlying wheat's differential sensitivity to HPPD-inhibitors, providing insights that may support breeding or genetic engineering of herbicide-tolerant wheat in the future. © 2025 Society of Chemical Industry.
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