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Related Experiment Video

Updated: Sep 16, 2025

Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain
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Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain

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Deep-tissue two-photon brain imaging enabled by a tunable fiber-optic dispersive wave generator.

Marvin Edelmann1,2,3, Andreu Matamoros-Angles4, Mohsin Shafiq4

  • 1Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607, Hamburg, Germany. marvin.edelmann@desy.de.

Scientific Reports
|July 8, 2025
PubMed
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We developed a fiber-optic system for efficient, tunable ultrashort pulse generation. This breakthrough enables multicolor deep-tissue two-photon imaging of brain structures with unprecedented detail and efficiency.

Area of Science:

  • Biomedical Optics
  • Ultrafast Laser Technology
  • Neuroimaging

Background:

  • Two-photon imaging offers high resolution for deep-tissue visualization.
  • Generating tunable, high-energy ultrashort pulses is crucial for advanced imaging techniques.
  • Existing methods often face limitations in efficiency and tunability.

Purpose of the Study:

  • To develop a novel fiber-optic dispersive wave generator.
  • To achieve highly efficient, wavelength-tunable ultrashort pulse generation.
  • To enable multicolor deep-tissue two-photon imaging of neuronal and vascular structures.

Main Methods:

  • Utilized a compact Yb: fiber laser-driven system.
  • Employed precisely parameter- and phase-matching-controlled dispersive wave generation in photonic crystal fiber.

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Last Updated: Sep 16, 2025

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  • Guided system construction with comprehensive numerical simulations.

    • Main Results:

    • Generated sub-100 fs pulses with over 6.7 nJ energy.
    • Achieved a continuously tunable spectral range of 880-950 nm.
    • Attained a record-high optical conversion efficiency of up to 65%.

    Conclusions:

    • The developed system enables high-resolution structural imaging in mouse brain at depths exceeding 450 μm.
    • Optimized output for two-photon excitation of common fluorescent proteins.
    • Represents a significant advancement in ultrafast fiber laser technology for biomedical imaging.