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Updated: Sep 16, 2025
A Unified Methodological Framework for Vestibular Schwannoma Research
Published on: June 20, 2017
Integrating single-cell and spatial transcriptomics reveals the cellular heterogeneity of vestibular schwannoma
Wenqi Dong1, Yuchen Jin1, Lingkang Dong1
1Shanghai Key Laboratory of Sleep Disordered Breathing, Department of Otolaryngology-Head and Neck Surgery, Otolaryngology Institute of Shanghai Jiao Tong University, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Abstract:
Vestibular schwannoma (VS) is a benign tumor that can result in significant neurological and otological complications. The mechanisms underlying its development and spatial heterogeneity remain poorly understood. In this study, we analyzed single-cell RNA sequencing (scRNA-seq) data from three previously published vestibular schwannomas (VS1-3), along with spatial transcriptomics data from two additional specimens (VS_S1-2). Our results identified a VEGFA-enriched Schwann cell (SC) subtype in scRNA-seq data, which was validated by spatial transcriptomics. This subtype also exhibited a significant positive correlation with NOVhi SCs expression. These cells were centrally localized within tumor tissue. Furthermore, spatial analysis provided new insights into SC-stromal cell interactions, and we constructed a preliminary cellular atlas of VS tissues, enhancing our understanding of tumor growth dynamics.
