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Updated: Sep 16, 2025

Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins

Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins

Published on: January 13, 2023

Progress in Dried Microsampling for MS-Based Protein Analysis.

Trine Grønhaug Halvorsen¹, Léon Reubsaet¹

¹Department of Pharmacy, University of Oslo, Oslo, Norway.

Journal of Separation Science

|July 9, 2025

Summary

This summary is machine-generated.

Dried microsampling for mass spectrometry (MS) protein analysis shows rapid clinical progress, enabling targeted biomarker discovery and noninvasive lifestyle monitoring. Innovations like smart sampling address challenges, enhancing precision for future applications.

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Last Updated: Sep 16, 2025

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Area of Science:

Biochemistry
Analytical Chemistry
Biotechnology

Background:

Dried microsampling offers a minimally invasive approach for biological sample collection.
Mass spectrometry (MS) is a powerful technique for protein analysis.
Recent advancements have expanded the utility of dried microsamples in proteomics.

Purpose of the Study:

To review recent progress (2020-January 2025) in dried microsampling for MS-based protein analysis.
To highlight clinical applications, biomarker discovery, and technological innovations.
To discuss challenges and future directions in the field.

Main Methods:

Systematic literature search for studies on dried microsampling and MS protein analysis.
Analysis of applications in clinical diagnostics, proteomics, and lifestyle monitoring.
Review of sample quality, alternative matrices, and innovative sampling techniques.

Main Results:

Clinical applications dominate, focusing on targeted biomarker determination using multiplex methods.
Non-targeted proteomics screenings identify new biomarkers for disease diagnostics and lifestyle monitoring.
Smart sampling innovations simplify preparation and improve analytical precision.

Conclusions:

Dried microsampling is increasingly vital in clinical and research settings for MS protein analysis.
Continued technological advancements are expected to broaden its adoption and utility.
Future innovations will likely further enhance sample preparation and analytical capabilities.