Treatment targets of cellular senescence and circadian rhythms in ischemic stroke

Ischemic stroke poses a substantial public health burden due to its high incidence, rendering it an urgent medical concern. Emerging evidence suggests that cellular senescence and circadian rhythms are closely linked to the onset of cerebral infarction and may represent promising therapeutic targets. Accordingly, we retrieved datasets GSE16561 and GSE22255 from the Gene Expression Omnibus and extracted cellular senescence- and circadian rhythm-related genes from the GeneCards database. Through bioinformatics analysis, we identified key targets associated with cellular senescence and circadian rhythm in cerebral infarction, specifically CREBBP, FOS, CDK4, MMP9, PTEN, and HIF1A. Complementing these findings with in vitro experiments, our results demonstrate that the expression of these genes and proteins at these core targets are elevated to varying degrees in an in vitro model of cerebral infarction. These findings provide compelling evidence supporting the pivotal role of cellular senescence- and circadian rhythm-related genes in the pathogenesis of ischemic stroke.