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Updated: Sep 16, 2025
T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
Published on: January 12, 2021
T-Cell Receptor Repertoires Show Dynamic Variation Between Diagnosis and Relapse of Diffuse Large B-Cell Lymphoma
Joel Wight1,2,3,4, Tom Witkowski2, Colm Keane5,6
1Austin Health Heidelberg Australia.
Background:
Tumour infiltrating lymphocyte (TIL) T-cell receptor (TCR) repertoire is prognostic in newly diagnosed diffuse large B-cell lymphoma (DLBCL), but evolution has not been evaluated at relapse.
Methods:
We examined the TCR repertoire in nine paired DLBCL samples from diagnosis and relapse.
Results:
We noted considerable differences, with dominant clones at diagnosis replaced at relapse by new clones that were absent or minor initially. There was low linearity between diagnostic and relapsed samples (r-values 0.01-0.316), with shared clones averaging 8.3% (range 0%-37%). Clonal diversity was reduced in relapsed samples, suggesting an increasingly defunct intratumoural immune response.
Conclusion:
T-cell diversity is reduced in relapsed/refractory DLBCL, which may have implications for immunotherapy usage.
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