Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Plasmodesmata

Plasmodesmata

The organs in a multicellular organism’s body are made up of tissues formed by cells. To work together cohesively, cells must communicate. One way that cells communicate is through direct contact with other cells. The points of contact that connect adjacent cells are called intercellular junctions.

The Apoplast and Symplast

The Apoplast and Symplast

Plant growth depends on its ability to take up water and dissolved minerals from the soil. The root system of every plant is equipped with the necessary tissues to facilitate the entry of water and solutes. The plant tissues involved in the transport of water and minerals have two major compartments - the apoplast and the symplast. The apoplast includes everything outside the plasma membrane of living cells and consists of cell walls, extracellular spaces, xylem, phloem, and tracheids. The...

Contact-dependent Signaling

Contact-dependent Signaling

Contact-dependent signaling, as the name suggests, requires that communicating cells be in direct contact with each other. This is achieved either through receptor-ligand interactions or by specialized cytoplasmic channels that allow the flow of small molecules between cells. In animal cells, channels called gap junctions facilitate contact-dependent signaling in certain tissues, whereas, plasmodesmata perform a similar function in plants.
Gap Junctions
In animal cells, gap junctions are formed...

Cell Adhesion in Plants

Cell Adhesion in Plants

Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...

Short-distance Transport of Resources

Short-distance Transport of Resources

Short-distance transport refers to transport that occurs over a distance of just 2-3 cells, crossing the plasma membrane in the process. Small uncharged molecules, such as oxygen, carbon dioxide, and water, can diffuse across the plasma membrane on their own. In contrast, ions and larger molecules require the assistance of transport proteins due to their charge or size. Transport across membranes also occurs within individual cells, playing a variety of essential roles for the plant as a whole.

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Attenuation of cell-cell movement of tobamoviruses by the C-terminal GPI signal peptide of arabinogalactan protein FLA8.

Current biology : CB·2026

Same author

A broad-spectrum anti-fungal effector dictates bacterial-fungal interkingdom interactions.

PLoS pathogens·2025

Same author

Bacterial Acetyltransferase Effector AopP2 Primes Effector-Triggered Immunity in Watermelon by Acetylating a Conserved Transcription Factor.

Plant, cell & environment·2025

Same author

Fha initiates the inside-out assembly of the type VI secretion system.

Cell reports·2025

Same author

Rhizobium tropici biopolymer enhances lateral root formation in agar-grown Arabidopsis.

Biochemical and biophysical research communications·2025

Same author

The roles of movement and coat proteins in the transport of tobamoviruses between plant cells.

Frontiers in plant science·2025

Search research articles

Related Experiment Video

Updated: Sep 16, 2025

Author Spotlight: Microscopic Analysis of Protein Localization at Plasmodesmata in Plants

Author Spotlight: Microscopic Analysis of Protein Localization at Plasmodesmata in Plants

Published on: November 1, 2024

PDLP5, a plasmodesmata permeability regulator, can traffic between plant cells.

Yumin Kan¹, Vitaly Citovsky¹

¹Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY USA.

NPJ Science of Plants

|July 9, 2025

Summary

Plasmodesmata proteins like PDLP5 can move between plant cells, a process essential for intercellular communication. This cell-to-cell movement of PDLP5 involves its transmembrane domain, offering new insights into its function.

Keywords:

Plant cell biology Plant molecular biology

More Related Videos

Identification of Plasmodesmal Localization Sequences in Proteins In Planta

Identification of Plasmodesmal Localization Sequences in Proteins In Planta

Published on: August 15, 2017

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response

Published on: April 17, 2016

Related Experiment Videos

Last Updated: Sep 16, 2025

Author Spotlight: Microscopic Analysis of Protein Localization at Plasmodesmata in Plants

Author Spotlight: Microscopic Analysis of Protein Localization at Plasmodesmata in Plants

Published on: November 1, 2024

Identification of Plasmodesmal Localization Sequences in Proteins In Planta

Identification of Plasmodesmal Localization Sequences in Proteins In Planta

Published on: August 15, 2017

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response

Published on: April 17, 2016

Area of Science:

Plant cell biology
Molecular plant pathology

Background:

Plasmodesmata (PD) are crucial for plant intercellular communication, regulating the transport of molecules and pathogens.
PD-located proteins (PDLPs) and PD callose binding proteins (PDCBs) are key regulators of PD pore size.
The cell-to-cell mobility of PDLP proteins themselves is largely unknown.

Purpose of the Study:

To investigate the cell-to-cell movement capacity of PDLP proteins.
To determine if the transmembrane domain of PDLP5 is involved in its intercellular trafficking.

Main Methods:

Expression of PDLP1, PDLP2, and PDLP5 in *Nicotiana benthamiana*.
Confocal microscopy to visualize protein localization and movement.
Analysis of PDLP5 movement dependent on its native transmembrane domain.

Main Results:

PDLP5 demonstrated efficient cell-to-cell movement in *Nicotiana benthamiana*.
PDLP1 and PDLP2 did not exhibit significant cell-to-cell movement.
The native transmembrane domain of PDLP5 was found to be essential for its cell-to-cell trafficking.

Conclusions:

PDLP5 possesses the ability to traffic between plant cells, unlike PDLP1 and PDLP2.
The transmembrane domain of PDLP5 plays a critical role in its intercellular movement.
These findings enhance understanding of PDLP5's complex role in plasmodesmata function and transport.