Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Spatially resolved transcriptome-metabolome integration reveals region-specific glial lipid dysregulation associated with Alzheimer's pathology.

bioRxiv : the preprint server for biology·2026

Same author

PURE-seq integrates FACS and PIP-seq for single-cell genomics of ultra-rare cells.

Nature communications·2026

Same author

scGALA advances graph link prediction-based cell alignment for comprehensive data integration and harmonization.

Nature communications·2025

Same author

Deterministic cell pairing with simultaneous microfluidic merging and sorting of droplets.

Lab on a chip·2025

Same author

Tumor heterogeneity underlies clinical outcome and MEK inhibitor response in somatic NF1-mutant glioblastoma.

JCI insight·2025

Same author

Myeloid-mesenchymal crosstalk drives ARG1-dependent profibrotic metabolism via ornithine in lung fibrosis.

The Journal of clinical investigation·2025

Search research articles

Related Experiment Video

Updated: Jun 17, 2026

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

Published on: February 11, 2019

Deterministic Cell Pairing with Simultaneous Microfluidic Merging and Sorting of Droplets.

Kevin M Joslin¹, Sophia Dateshidze¹, Seung Won Shin¹

¹Department of Bioengineering, University of California, Berkeley, California Institute for Quantitative Biosciences, Berkeley, California, USA.

Biorxiv : the Preprint Server for Biology

|July 9, 2025

Summary

Researchers developed a microfluidic device for precise cell pairing, enabling high-throughput studies of cell crosstalk. This merge-sort technology significantly improves the recovery of desired cell combinations for biological research.

More Related Videos

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

Published on: September 27, 2019

Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology

Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology

Published on: December 1, 2023

Related Experiment Videos

Last Updated: Jun 17, 2026

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

Published on: February 11, 2019

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

Published on: September 27, 2019

Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology

Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology

Published on: December 1, 2023

Area of Science:

Biotechnology
Microfluidics
Cell Biology

Background:

Cell-cell interactions are crucial for biological processes like immune response and tissue repair.
Studying cell crosstalk requires methods to create defined cell pairs, which are currently limited.
High-throughput droplet microfluidics often suffers from random cell loading, yielding low percentages of desired combinations.

Purpose of the Study:

To develop a microfluidic device for deterministic isolation of specific cell pairs.
To enable high-throughput studies of cell crosstalk using precise cell co-culture.
To overcome the limitations of random loading in droplet microfluidics.

Main Methods:

A microfluidic device utilizing droplet merging and sorting ('merge-sorting') was developed.
The system employs fluorescence detection to identify target cell combinations.
Simultaneous electrocoalescence and dielectrophoretic sorting are triggered for isolation.

Main Results:

Achieved 98.6% purity of merged and sorted fluorescent dye-loaded droplets.
>90% recovery of desired cell pairs, a significant improvement over <1% with random loading.
Produced monodisperse alginate hydrogels with 92% target cell pairs, maintaining viability for 18 hours.

Conclusions:

The merge-sort platform enables flexible enrichment of specific droplet and cell combinations for high-throughput cell crosstalk studies.
This method offers superior purity and recovery compared to existing techniques, facilitating cleaner downstream workflows.
The device supports pre-incubation, multi-step assays, and isolation of desired pairs, offering versatile capabilities for biological research.