Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Homologous Recombination02:31

Homologous Recombination

52.4K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
52.4K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

12.9K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
12.9K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.9K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.9K
Mismatch Repair01:36

Mismatch Repair

40.6K
Overview
40.6K
DNA Damage can Stall the Cell Cycle02:37

DNA Damage can Stall the Cell Cycle

9.3K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.3K
The DNA Replication Fork01:02

The DNA Replication Fork

36.9K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
36.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multiplex engineering of rhesus macaque NK cells enhances homing to sites of HIV replication in B cell follicles.

bioRxiv : the preprint server for biology·2026
Same author

Rap1-mediated steric hindrance protects telomeres from MRX sensing.

Nature structural & molecular biology·2026
Same author

Adenine base editor for knockout of proteins: A practical guide from design to analysis with updated MultiEditRbatch.

Molecular therapy. Nucleic acids·2026
Same author

Efficient multiplex non-viral engineering and expansion of polyclonal γδ CAR-T cells for immunotherapy.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

CISH, a key intracellular checkpoint, in comparison and combination to existing and emerging cancer immune checkpoints.

Communications biology·2026
Same author

Visualization and quantification of rDNA instabilities in mammalian cells and mouse models.

Nucleic acids research·2026

Related Experiment Video

Updated: Sep 16, 2025

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

10.4K

Asymmetrical recognition and processing of double-strand breaks formed during DNA replication.

Matthew J Johnson, Michael T Kimble, Seoyeong Jeong

    Biorxiv : the Preprint Server for Biology
    |July 9, 2025
    PubMed
    Summary

    DNA double-strand break (DSB) repair differs at replication-dependent breaks. Mre11 protein preferentially binds blunt ends, while 3' overhangs allow Mre11-independent resection, impacting non-homologous end joining repair.

    More Related Videos

    Author Spotlight: Visualizing Single-Stranded DNA During DNA Repair for Therapeutic Insights
    08:30

    Author Spotlight: Visualizing Single-Stranded DNA During DNA Repair for Therapeutic Insights

    Published on: December 22, 2023

    2.7K
    Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
    06:44

    Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

    Published on: April 28, 2021

    4.1K

    Related Experiment Videos

    Last Updated: Sep 16, 2025

    Visualization of DNA Repair Proteins Interaction by Immunofluorescence
    07:55

    Visualization of DNA Repair Proteins Interaction by Immunofluorescence

    Published on: June 26, 2020

    10.4K
    Author Spotlight: Visualizing Single-Stranded DNA During DNA Repair for Therapeutic Insights
    08:30

    Author Spotlight: Visualizing Single-Stranded DNA During DNA Repair for Therapeutic Insights

    Published on: December 22, 2023

    2.7K
    Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
    06:44

    Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

    Published on: April 28, 2021

    4.1K

    Area of Science:

    • Molecular Biology
    • DNA Repair Mechanisms
    • Cell Cycle Regulation

    Background:

    • DNA end resection is crucial for homology-directed double-strand break (DSB) repair.
    • Resection at replication-dependent DSBs remains poorly understood compared to endonuclease-induced DSBs.

    Purpose of the Study:

    • To investigate the mechanism of DNA end resection at replication-dependent DSBs.
    • To elucidate the roles of Mre11, Ku, Exo1, and Dna2-Sgs1 in processing these breaks.

    Main Methods:

    • Utilized a Cas9D10A nickase system in budding yeast to create replication-dependent DSBs.
    • Analyzed DNA break end structures and protein binding preferences (Mre11, Ku).
    • Investigated resection pathways (Mre11-dependent vs. Mre11-independent) involving Exo1 and Dna2-Sgs1.

    Main Results:

    • Replication-dependent DSBs exhibit asymmetric processing, with one blunt/near-blunt end and one 3' ssDNA overhang.
    • Mre11 preferentially binds blunt ends and facilitates Ku removal.
    • 3' overhang ends show minimal Ku binding, enabling Mre11-independent resection via Exo1 or Dna2-Sgs1.

    Conclusions:

    • DNA end resection differs significantly between replication-dependent and canonical DSBs.
    • Ku selectively binds nearly blunt ends, potentially explaining the inefficient non-homologous end joining repair of replication-dependent DSBs.