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Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
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A comparative analysis of fixatives for improved histological integrity in the human ovary†

Elizabeth Varghese1, Mary E Soliman1, Hong Lou1

  • 1Department of Pediatric and Adolescent Gynecology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD, USA.

Biology of Reproduction
|July 9, 2025
PubMed
Abstract

No abstract available in PubMed .

Keywords:
fertility preservationhistologymodified Davidson’s fixative (mDF)neutral buffered formalin (NBF)ovarian folliclesovarian tissue cryopreservation (OTC)paraformaldehyde (PFA)tissue fixation

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
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