Related Experiment Video
Updated: Sep 16, 2025
05:39
Detection of MicroRNA Expression in the Kidneys of Immunoglobulin A Nephropathic Mice
Published on: July 8, 2020
1.9K
Identification of Renal Transcripts Associated with Kidney Function and Prognosis in ANCA-Associated Vasculitis
Benoît Brilland1,2,3, Jérémie Riou4, Thomas Quéméneur5
1Service de Néphrologie-Dialyse-Transplantation, CHU Angers, Angers, France.
Journal of the American Society of Nephrology : JASN
|July 9, 2025
Summary
A new 12-gene signature from kidney transcriptomics accurately predicts kidney survival in ANCA-associated vasculitis with glomerulonephritis (AAV-GN). This molecular approach surpasses current risk stratification tools for better patient outcomes.
Area of Science:
- Nephrology
- Immunology
- Genomics
Background:
- ANCA-associated vasculitis with glomerulonephritis (AAV-GN) often leads to kidney failure, with limited tools for predicting outcomes.
- Current risk stratification methods do not fully capture kidney injury's molecular complexity, hindering personalized treatment.
Purpose of the Study:
- To investigate if kidney transcriptomics can identify molecular signatures associated with kidney outcomes in AAV-GN.
- To develop a gene-based prognostic signature for improved risk stratification.
Main Methods:
- Analyzed kidney biopsies from 199 AAV-GN patients and 23 controls using NanoString nCounter for 750 immune-related genes.
- Performed differential gene expression, pathway enrichment, and immune cell infiltration analyses.
- Developed and validated a 12-gene prognostic signature using LASSO-penalized Cox regression.
Main Results:
- AAV-GN showed significant immune dysregulation, with 150 differentially expressed genes and altered immune cell infiltration.
- A 12-gene signature demonstrated a stronger association with kidney survival than established histological classifications (p < 0.0001).
- Specific immune pathway expressions, particularly lymphocyte trafficking, correlated with patient outcomes.
Conclusions:
- Kidney biopsy transcriptomics reveals key immune-related genes in AAV-GN.
- A novel 12-gene signature accurately predicts kidney survival in AAV-GN patients.
- This molecular signature offers superior risk stratification compared to existing methods.
Related Concept Videos
01:19Imaging Studies VII: Vascular Imaging
62
DefinitionRenal angiography, also known as renal arteriography, is an imaging technique used to obtain a comprehensive view of blood flow and the vascular structure of blood vessels in the kidneys and surrounding areas.PurposeRenal angiography detects blood vessel abnormalities in the kidneys, such as aneurysms, stenosis, thrombosis, vascular tumors, and renal artery stenosis. It evaluates kidney function and guides interventional treatments like angioplasty or stent placement.Pre-Procedure...
62
01:30Acute Kidney Injury IV: Diagnostic Studies and Prevention
59
Accurate diagnosis and effective prevention are critical in managing Acute Kidney Injury (AKI), which is linked to high mortality rates ranging from 10% to 80%. Timely recognition of at-risk patients and careful monitoring can significantly reduce the likelihood of kidney damage.Diagnostic Assessments:The diagnostic process starts with a comprehensive medical history to identify prerenal, intrarenal, and postrenal causes.Prerenal causes, such as dehydration, hypotension, or blood loss, should...
59
01:29Acute Kidney Injury II: Pathophysiology
92
Acute kidney injury (AKI) causes are categorized into three primary categories based on the location of the injury: prerenal, intrarenal (or intrinsic), and postrenal causes. This classification guides clinical management and illustrates how different pathways can impair kidney function.Etiology and Pathophysiology of Acute Kidney Injury1. Prerenal causesEtiology: Prerenal Acute Kidney Injury, the most common type, occurs when reduced blood flow to the kidneys decreases filtration capacity...
92
01:20Renal Corpuscle
3.3K
The glomerulus and Bowman's capsule are two essential components of the nephron, which is the functional unit of the kidney. These microscopic structures play a critical role in the process of blood filtration to produce urine.
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous...
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous...
3.3K
01:10Nephrons
3.5K
The kidneys are intricate organs with millions of working units known as nephrons. Each nephron features two major structures: the renal corpuscle, which facilitates blood plasma filtration, and the renal tubule, which handles the glomerular filtrate. Blood supply is directly linked to the nephrons. The renal corpuscle consists of the glomerulus, a capillary network, and the Bowman's capsule, a double-walled epithelial structure that encases the glomerulus. The filtering of blood plasma...
3.5K