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Multifunctional AuAgPt Nanoframes for a Stimuli-Responsive Electrochemiluminescence Aptasensor
Changxiao Song1, Jingxian Li1, Futing Wang1
1Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Material Science and Engineering, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha, Hunan 410082, China.
This study introduces a novel electrochemiluminescence (ECL) aptasensor for detecting aflatoxin B1 (AFB1). The sensor utilizes multifunctional gold-silver-platinum nanoframes (AuAgPt NFs) for highly sensitive and rapid AFB1 detection.
Area of Science:
- Analytical Chemistry
- Materials Science
- Biotechnology
Background:
- Aflatoxin B1 (AFB1) is a potent carcinogen requiring sensitive detection methods.
- Existing electrochemiluminescence (ECL) sensors face challenges in sensitivity and signal amplification.
- Nanomaterials offer unique properties for enhancing biosensor performance.
Purpose of the Study:
- To develop a novel multifunctional electrochemiluminescence (ECL) coreaction accelerator for enhanced aflatoxin B1 (AFB1) detection.
- To design a highly sensitive ECL aptasensor for AFB1 using gold-silver-platinum nanoframes (AuAgPt NFs).
- To investigate the mechanism of ECL-RET quenching and the role of AuAgPt NFs as a coreaction accelerator.
Main Methods:
- Synthesis of AuAgPt nanosheets (NSs) and their transformation into nanoframes (NFs).
- Fabrication of an ECL aptasensor incorporating g-C3N4@Au and AuAgPt NFs.
- Utilizing ECL resonance energy transfer (ECL-RET) for signal quenching and coreaction acceleration.
- Employing an Ag-dependent DNAzyme and AFB1 aptamer for specific analyte recognition.
Main Results:
- AuAgPt NSs initially quenched the ECL signal via ECL-RET.
- Hydrogen peroxide (H2O2) treatment transformed NSs into NFs, disrupting ECL-RET and enhancing ECL signals.
- The AFB1 aptasensor demonstrated high sensitivity with a limit of detection (LOD) of 0.11 fg/mL for AFB1.
- The sensor operated effectively within a wide detection range (1 fg/mL to 1 μg/mL).
Conclusions:
- The developed AuAgPt NFs serve as an effective multifunctional coreaction accelerator for ECL sensing.
- The proposed ECL aptasensor offers a highly sensitive and reliable platform for AFB1 detection.
- This approach holds promise for the rapid and accurate monitoring of mycotoxins in food safety applications.
