Enzyme immobilization based on reticular framework materials: Strategy, food applications, and prospect

Area of Science:

• Biocatalysis and Materials Science
• Focuses on enzyme immobilization using advanced porous materials.

Background:

• Natural enzymes offer high efficiency but suffer from poor stability and reusability.
• Immobilized enzyme technology addresses these limitations, enhancing enzyme performance and application scope.

Purpose of the Study:

• To comprehensively review strategies for enzyme immobilization on reticular framework materials (RFMs).
• To summarize research highlights and applications of RFM-based enzyme immobilization, particularly in the food industry.
• To identify future challenges and perspectives in this field.

Main Methods:

• Summarizes various immobilization strategies: physical adsorption, covalent linkage, diffusion, and in situ encapsulation.
• Reviews research on multi-enzyme systems, magnetic RFMs, and enzyme-mimicking RFMs.
• Compiles applications in the food industry.

Main Results:

• Reticular framework materials (RFMs) like MOFs, COFs, and HOFs offer high surface area, tunable porosity, and ordered structures ideal for enzyme carriers.
• Diverse immobilization techniques enable tailored enzyme integration onto RFMs.
• RFM-based enzyme immobilization shows promise for multi-enzyme systems and magnetic applications.

Conclusions:

• Enzyme immobilization on RFMs significantly improves enzyme stability and reusability, overcoming limitations of free enzymes.
• RFM-based systems present versatile platforms for advanced biocatalysis with broad industrial potential, especially in food processing.
• Further research is needed to address current challenges and unlock the full potential of RFM-enzyme conjugates.