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Updated: Jul 9, 2026

Capsular Serotyping of Streptococcus pneumoniae Using the Quellung Reaction
Published on: February 24, 2014
Carbapenem-resistant Acinetobacter baumannii isolate BAL114 from Vietnam produces capsular polysaccharide with two
Nikolay P Arbatsky1, Anastasiya A Kasimova2, Alexander S Shashkov1
1N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Abstract:
Capsular polysaccharide (CPS) produced by two Acinetobacter baumannii isolates carrying the KL58 locus were recently determined and found to differ due to the presence of additional CPS biosynthesis genes found in prophage. Here, we have examined the CPS produced by a third KL58-carrying A. baumannii isolate, BAL114, which was recovered in 2009 in Vietnam. CPS extracted from BAL114 was studied by sugar analysis, selective solvolysis with trifluoroacetic acid in the presence of 2-methyl-1-propanol, and Smith degradation along with one- and two-dimensional 1H and 13C NMR spectroscopy. Consistent with the genetic content of KL58, the polysaccharide was found to include tetrasaccharide K58 units consisting of a 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic (di-N-acetylpseudaminic) acid, d-Gal, d-Glc, and d-GalNAc. However, the linkage between the K58 units was different to the β-d-GalNAc-(1→3)-d-Glc linkage seen previously for other K58 forms. There were two CPS types in the ratio ∼1 : 1 with K58 units linked by either β-(1→3) or β-(1→4) linkages between the d-GalNAc and d-Gal residues.
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