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Published on: April 9, 2012
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Targeted next-generation sequencing for rapid tuberculosis detection: a systematic review and meta-analysis
Boya Gou1, Chuyao Zhang1, Zhe Song1
1National Clinical Research Center for Infectious Diseases, Shenzhen Third People's Hospital, Shenzhen 518112, China.
Summary
Targeted next-generation sequencing (tNGS) shows high accuracy for tuberculosis diagnosis. Its performance varies by sample type and sequencing platform, suggesting optimized application for better patient management.
Area of Science:
- Medical Diagnostics
- Genomic Sequencing
- Infectious Disease Research
Background:
- Targeted next-generation sequencing (tNGS) is a WHO-endorsed tool for tuberculosis diagnosis.
- Comprehensive evaluation of tNGS performance across diverse clinical scenarios is lacking.
Purpose of the Study:
- To investigate the diagnostic performance of tNGS for tuberculosis.
- To evaluate how clinical scenarios (populations, sample/tuberculosis types, platforms) influence tNGS accuracy.
Main Methods:
- Systematic literature search of multiple databases (PubMed, MEDLINE, etc.) for studies from 2005-2024.
- Quality assessment using QUADAS-2 tool and calculation of diagnostic accuracy metrics (sensitivity, specificity).
- Meta-analysis of 16 studies including 2,565 participants.
Main Results:
- Overall pooled sensitivity: 0.86, specificity: 0.95.
- Highest sensitivity observed with bronchoalveolar lavage fluid (0.91) and nanopore sequencing (0.85).
- tNGS showed significantly higher detection rates than GeneXpert MTB/RIF (OR: 1.78).
Conclusions:
- tNGS exhibits high diagnostic accuracy for tuberculosis.
- Performance is influenced by sample type and sequencing platform.
- Findings support optimizing tNGS for specific clinical settings to improve tuberculosis management.
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