Microfluidic electro-viscoelastic manipulation of extracellular vesicles

Microfluidic technology has created new opportunities for developing innovative tools for biological applications. Given the significance of extracellular vesicles (EVs), extensive research has focused on developing microfluidic techniques for EV isolation. This research protocol presents electro-viscoelastic microfluidics as a novel approach for manipulating EVs. The system leverages the viscoelasticity of the suspending medium along with an externally applied electric field to alter EV motion within a microchannel. These findings suggest that our electro-viscoelastic microfluidic system has the potential for further development to be used for EV isolation.