Related Experiment Video
Updated: Sep 16, 2025
Tumor Hypoxia Assessment: In Vivo 3D Oxygen Imaging Through Electron Paramagnetic Resonance
Published on: February 14, 2025
Visualization of Hypoxic Tumors: Stimulated by NIR-IIb Emissive Erbium(III)-Phthalocyaninate Nanoagent
Jingru Feng1, Weirui Zhu1, Wei Wei1
1MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangdong Provincial Key Laboratory of Laser Life Science, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510631, China.
Abstract:
This study presents a long-lived NIR-IIb luminogenic nanoagent (ErPc-Sa NPs) based on a newly designed erbium phthalocyaninate complex with impressive quantum yield at 1530 nm (Er, 2.81%) via single molecular self-assembly; NIR-IIb (1500-1700 nm) imaging holds tremendous potential for targeted screening of deep-seated pathological changes in tissues, though developing activatable NIR-IIb probes has been challenging. The luminescence of the nanoagent is switched on at 1530 nm upon activation by carbonic anhydrase (CA), a hypoxic tumor marker, under 680 nm excitation. ErPc-Sa NPs shows high specificity toward CA on MDA-MB-231 cell membranes under hypoxic conditions and the localized CA in tumor-bearing mice with NIR-IIb imaging.
