Effect of Andrographis paniculata and Melia azadirachta against the Dengue virus

Dengue infection is emerging as a global threat due to increased mortality and morbidity, especially in tropical and sub-tropical regions. Therefore, it is of interest to evaluate the antiviral activity of Andrographis paniculata and Melia azadirachta against Dengue virus-1 in a vero cell line using a cytopathic effects (CPE) reduction assay and cytotoxicity assessed by MTT assay. GC-MS analysis was conducted to identify phytochemicals in the plant extracts. Both extracts showed potent antiviral activity at 150 µg/ml and were non-toxic up to 1.5 mg/ml, with 100% cell viability. These findings suggest that A. paniculata and M. azadirachta are promising candidates against Dengue virus-1, warranting further investigation into their active compounds.